Abstract: Artemisia anethifolia was employed to study the relationship of salt tolerance with Soluble protein contents and Soluble carbohydrate contents in seedling of Artemisia anethifolia. The results showed that, with the increase of salt concentration, the seedling Soluble protein contents of Artemisia anethifolia had low-high-low trend; otherwise, Stem and Leaves of Artemisia anethifolia had low-high trend and roots had low-high-low trend. It was concluded that Soluble protein contents and Soluble carbohydrate contents could be used as the physiological indexes for salt tolerance in Artemisia anethifolia.