Abstract:

Cucumber green mottle mosaic virus in virus-infected cucumber leaves was detected by DAS-ELISA、RT-PCR and NASH techniques and the sensitivities of detection were compared after the total RNA extracted from 1g virus-infected leaves were diluted gradient 。The results showed that the detection of RT-PCR was the most sensitive; NASH was more sensitive than DAS-ELISA. NASH technique can be replaced of RT-PCR used in metrical quarantine departments by comparison of sensitivities of three detect techniques