Abstract:

Abstract:【Objective】 In a comprehensive analysis of a variety of wheat genomic DNA extraction methods, one easy and efficient wheat genomic DNA extraction method had been improved and developed.【Method】 Spring wheat cultivar Thatcher, TcLr19, TcLr20 and TcLr28 as the materials were used to extract the DNA. And then was used as template to amplify the STS marker for leaf rust resistance gene Lr20, SCAR marker for Lr19 and Lr28, SSR marker for Lr28. 【Result】The result showed that clear and right bands or smear were amplified. 【Conclusion】It means that the method could get the high quality DNA for PCR analysis. This method reduce the DNA extracting processing, costs, and pollution to the environment and human being, and therefore provided solid base for the preparation of large number of samples for PCR-based assays.