Abstract:

Abstract: For the purpose to explore whether differences exist in qSH1 gene between weedy rice and Oryza sativa. Using the primers that had been used to amplify the qSH1 gene of Oryza sativa, PCR was done with 6 samples of weedy rices origin from Zhanjiang of Guangdong province, the qSH1 gene fragments were sequenced, the result of sequences alignment showed that the nucleotide sequence locates at region approximately 100kb downstream of qSH1, and was homologous with what has collected on the NCBI website, the homology value was up to 100%. There was a nucleotide site difference between the 6 weedy rices and Oryza sativa, which was proved to be the SNP site that reported before. At the same time, not all the nucleotide sequences that derived from the 6 weedy rices are the same, there is a different nucleotide site, but can’t determine whether it is an action site.