Abstract:

The efficiency and the effect of five RNA isolation methods, including CTAB, SDS, modified CTAB, modified SDS and Trizol, were studied in the RNA extraction from apricot leaves and fruitlets. Of these five methods, the method of Trizol was found to be the best one, and the modified CTAB was better for the leaf RNA isolation. The RNA yields from apricot leaf were 839.25μg/g and 395.21μg/g, respectively. The modified CTAB and SDS methods were preferential ones for the isolation of higher quantity and better quality RNA from apricot fruit. All the RNA samples could be successfully used to amplify a specific cDNA fragment of UBQ, a housekeeping gene in plant, which suggests the utility of the RNA samples in cDNA library construction or gene cloning.