Abstract:

Abstract:【OBJECTIVE】Through determining the sensitivity of two strains of Botritis cinerea to six fungicides, screen fungicides which had a good fungicidal activity. 【METHOD】The six fungicides were tested by the methods of mycelial growth inhibition and spore germination inhibition, which were Procymidone, Iprodione, Pyrimethanil, Thiophanate-methyl, Diethofencarb and Carbendazim.【RESULTS】The results showed that the activity of Diethofencarb was the best, and Carbendazim had the weakest virulence to B. cinerea with its EC50 higher than 200 μg/ml. The strains SY and WS showed moderate and high resistance to Iprodione, respectively. The strain WS showed a high level resistance to Thiophanate-methyl，and both of the two strains had a high level resistance to Carbendazim. Significant difference between the results by two bioassay methods in Procymidone and Pyrimethanil was observed. Procymidone had obviously inhibited spore germination, while Pyrimethanil had a good activity on inhibiting mycelial growth.【CONCLUSION】 Diethofencarb was the best fungicide to inhibit B. cinerea. The frequency of use of Thiophanate-methyl and Carbendazim should be reduced.