Abstract:

Objective:Cloning and Expression of △6-Fatty acid Desaturase Gene From Mucor.pusillusv.Methods:The research used a strain of GLA produceing Mucor, through PCR method, got a △6-fatty acid desaturase gene of Mucor.pusillus,and generated a recombinant vector pYMpD6D.The recombinant plasmid pYMpD6D was transformed into S. cerevisiae INVScl using lithium acetate method, and using the yeast expresses system pYES2.0 to confirm that this gene can cause the yeast to synthesis GLA. Results:Though GC analyze, the GLA content about 6.53％ in total fatty acid.This research broaden a way for deep research on △6-fatty acid desaturase gene family,and lay certain foundation for further uses this gene to improve the crops quality.