Abstract:

The objective of this experiment is to evaluate the effect of calcium and JA on the expression of CBF in spinach. The seedlings of spinach were treated with low temperature (4℃), JA or A23187, one kinds of calcium ionophore respectively, then detected the expression of CBF by northern blotting. The results showed that low temperature and JA regulated the CBF expression positively. Low temperature may increase the JA content of the cell firstly, then JA induced the increase of cytosolic calcium concentration ([Ca2+]cyt), and the JA induced Ca2 + to transmit the low temperature signal through CaM or CaM related proteins, and regulated the CBF expression.