Abstract:

Take two type(watching type and wooding type) of softwood as explant, by studying the plant regeneration and rooting condition , to establish standardized in vitro propagation technology system for Sequoia sempervirens (Larnb.) Endl. The result showed that Sequoia sempervirens (Larnb.) Endl is a kind of woody plant multiplication easily and the inorganic salt concentration is the key factor for its rooting; their optimal multiplication medium is MS + 6-BA 0.1mg/L + NAA 0.1mg/L + KT0.1 mg/L, and the best rooting medium is 1/4MS + NAA 0.4 mg/L, the watching type rooting better than the wooding type.