Abstract:

Using different culture medium formula on the tissue culture experiment for Gerbera receptacle, a comprehensive analysis of the various culture medium formulas in the process of Gerbera tissue culture which effects the cultivation of callus induction, proliferation of successive transfer culture, strong sprout and rooting. It turns out that most appropriate tissue culture medium of Gerbera callus induction in the test formulations is MS + 6BA10mg/L + NAA1.0mg/L + sugar 30g/L + agar 5%; the most appropriate tissue culture medium for the proliferous cultivation of successive generations is MS + 6BA1.0mg/L + NAA0.2mg/L + sugar 30g/L + agar7% ; the most appropriate tissue culture medium of strong sprout and rooting is 1/2MS + NAA0.5mg/L + sugar20g/L+ agar 7%.