Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (12): 249-254.
Special Issue: 生物技术
• 23 • Previous Articles Next Articles
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To investigate the functions of PEPCase in lipid content, fatty acid composition and the response to environmental stress in C3 plants, Arabidopsis thaliana plant-type PEPCase genes (referred to as Atppc1, Atppc2 and Atppc3) were silence by the artificial miRNA (amiRNA) technique. We constructed the plant expression vector named pFGC-amiAtppc123 and transformed it into Arabidopsis thaliana via in floral dip method. Transgenic plants were confirmed by PCR. The amiRNA transcript levels were detected by semi-quantitative RT-PCR. The results showed that amiRNA expression was drastically increased in transgenic plant compared with non-transgenic plants.
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https://www.casb.org.cn/EN/Y2011/V27/I12/249