Abstract:

To investigate the functions of PEPCase in lipid content, fatty acid composition and the response to environmental stress in C3 plants, Arabidopsis thaliana plant-type PEPCase genes (referred to as Atppc1, Atppc2 and Atppc3) were silence by the artificial miRNA (amiRNA) technique. We constructed the plant expression vector named pFGC-amiAtppc123 and transformed it into Arabidopsis thaliana via in floral dip method. Transgenic plants were confirmed by PCR. The amiRNA transcript levels were detected by semi-quantitative RT-PCR. The results showed that amiRNA expression was drastically increased in transgenic plant compared with non-transgenic plants.