Abstract:

Abstract: In the present study, regulative effects of bufonis parenteral sloution on ?orfenicol-induced immunosuppression in mice were evaluated. Animal model of ?orfenicol-induced immunosuppression was made. Slpeens were weighted and calculated weight index. OVA-speci?c IgG, IgG1, IgG2b were measured by ELISA，splenocytes were harvested for determining lymphocyte proliferation and spleen lymphocyte secrete cytokines were measured by ELISA method. The results showed that bufonis parenteral solution could increase the weight and the level of antibody. Bufonis parenteral solution treated combined with polyclonal T cell mitogen (Con A or LPS) in a certain dose range, the effects significantly enhanced the proliferation. Bufonis parenteral solution also induce cytokines secretion in vivo. The results indicated that bufonis parenteral solution possessed the regulative effects on ?orfenicol-induced immunosuppression in mice.