Abstract:

To establish a HPLC method for the determination of sucrose synthase and sucrose phosphate synthase activity in Rehmannia glutinosa by HPLC, the activity of sucrose synthase and sucrose phosphate synthase was calculated through detecting the changes of sucrose content by HPLC. The column was Waters XBridgeTM Amide 3.5 ?m (4.6×150 mm Column)，with acetonitrile-water (70:30) as mobile phase, at a flow rate of 0.5 mL/min and a column temperature of 25℃. ELSD condition was optimized at the drift tube temperature of 40℃, the atomization gas of 30 psi and the spray mode of cooling. The regression line was linear over the concentration range of 0.128-3.2 μg (r=0.9999). The RSD by HPLC method was less than 5%. This method is stable and reliable, and has good precision and reproducibility for the determination of sucrose synthase and sucrose phosphate synthase Activity in Rehmannia glutinosa.