Abstract:

NAC transcription factors that are unique to plants are the new type transcription regulatory factors with multiple biological functions. Their family proteins contain a conserved amino acid sequence in N-terminal ends and highly different transcription regulatory domain in C terminal ends. In this experiment, a pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, tomato and Chili. NAC transcription factor gene fragment about 440 bp was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector, then the recombinant clones were sequenced. The sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. The results showed that NAC transcription factor gene gained from Cucurbita moschata included 442 bp, encoding 147 amino acids, which named CmNAC. The NAC gene fragments contain a conserved region like other plant NAC genes and belong to the NAC family ATAF1/2 subfamily.