Abstract:

The effects of explants type, hormone levels, casein hydrolysate on the growth of callus were measured by tissue culture. Meanwhile, the contents of total flavonoids and sophoridine in callus of different subculture times were determined by ultraviolet spectrophotometry and HPLC. The results showed that the cotyledon was optimum explants of callus induction, the best medium of callus induction was MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L+NAA 0.5 mg/L, and the best subculture medium was MS+2,4-D 1.0 mg/L+6-BA4.0 mg/L+NAA 1.0 mg/L. Contents of total flavonoids and sophoridine in callus of fifth subculture had the maximum, and 0.53 and 0.0112 respectively. So, the callus of fifth subculture was selected to use for material of cell suspension culture of S. alopecuroides.