Abstract:

Different bulks with 20, 25, 30, 35, 40, 45, 50, 55 plants were formed from an accession of 200 plants of a sesame landrace by randomly sampling. Genetic diversity of different bulks was analyzed by 15 pairs of EST-SSR markers at the DNA level. The results showed that the effective population size should be greater than 30-35 plants in order to represent the genetic characteristics of this germplasm, while it should be not less than 60-70 plants as appropriate population for reproduction of sesame landrace, to avoid gene drift and maintain the stability of genetic characteristics.