Abstract:

Two pretreatment methods which were SPE (solid-phase extraction) and SPME (solid-phase micro-extraction) had been studied to identify irradiated sunflower seeds by GC-MS (gas chromatography mass spectrum) analysis of six hydrocarbons, which were 1-C14:1 (1-tetradecene), C15:0 (n-pentadecane), 1-C16:1 (1-hexadecene), C17:0 (n-heptadecane), 1,7-C16:2 (1,7-hexadecadiene) and 8-C17:1 (8-heptadecene) between unirradiated and irradiated samples. Meanwhile, the effect of irradiation dose and frying process on hydrocarbons in sunflower seeds were also studied. Results showed that these two methods could both be used to identify whether sunflower seeds were irradiated or not, and HS-SPME-GC-MS method was easy to operate for quick and extract qualitative analysis. Irradiation dose had significant affected on hydrocarbons. Four hydrocarbons (1-C14:1, C15:0, 1-C16:1 and C17:0) were detected in unirradiated sunflower seeds. 1,7-C16:2 and 8-C17:1 were only detected in irradiated samples. Therefore, the two hydrocarbons were the best markers for detection of irradiated sunflower seeds.