Abstract:

The optimal conditions for the lywallzyme hydrolysis of the cultured monocaryon mycelia of Pleurotus nebrodensiss for protoplast production were investigate by single factor and orthogonal array design methods. The results showed that the highest production rate of the protoplast was emerged under the conditions that 7 days old liquid mycelium in the enzyme solution of 2% lywallzyme digested for 150 min at temperature of 27℃ with 0.6 mol/L mannitol as osmotic pressure stabilizer. Theoretical base for studying the protoplast fusion and transgenic of Pleurotus nebrodensiss was provided.