Abstract:

Collecting laccase from the fermentation liquid of auricularia and studying the enzymatic properties in the experiment can provide worthful theory data for application of laccase. Laccase liquid is prepared by (NH4)2SO4 fractional precipitation and dialysis. By using spectrophotometry and choosing ABTS [2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)], the activity of laccase can be determined. The results showed that when the laccase was insulated below 55℃, the relative activity and thermal stability was good. The optimal temperature was 45℃. It could be inhibited when the temperature was above 65℃. The optimal pH was 4.0. It could be inhibited when the pH was above 6.5. Metal ions could inhibit or enhance the activity of laccase.