Abstract:

12 AMT proteins were identified in grape genome by searching the protein database using Hidden Markov model (HMM) scan strategy. Using bioinformatics methods, genome localizations of 12 VvAMTs were analyzed firstly, and then predicted their amino acid composition, physical and chemical characteristics, as well as secondary structures. Meanwhile the relation of AMT gene family between Vitis vinifera, Arabidopsis thaliana, Oryza sativa and Populus trichocarpa was assayed. Genetic mapping of AMT genome localization found that they could be distributed on six chromosomes, which locations were more focus than their in Arabidopsis thaliana. 12 VvAMTs sequences could be divided into two sub-families, AMT1 sub-family had three members, and the rest belonged to AMT2 sub-family. The research still found that the amino acid number and sequence hydrophobic in 12 members of AMT gene family presented some differences. Furthermore, the main composition of 12 amino acid sequences were alpha helix and random coil via secondary structure predicted. Structural analysis of extrons and introns showed that only two members of VvAMT family had no intron, others had one, two, three or four introns. Protein subcellular localization analysis showed that all members of VvAMT were located in the membrane structure. EST analysis of VvAMT displayed that only five members had corresponding EST sequences, and only two members among them had a corresponding electronic expression profiles.