Abstract:

One bacterial strain of Aeramonas hydrophila (N201007) was isolated from affected Procambarus clarkii in anji area Zhejiang Province during an outbreak in 2010. The isolate could induce symptom of natural disease in ornamental Procambarus clarkii and caused death in 8 days，The semi-lethal LD50 to Procambarus clarkii was 5.3×105. Biological characterization showed that isolate was similar to A. hydrophila isolated in other aquatic animal. Analysis of 16S rRNA gene showed that the isolate shared more than 99 % identity with A. hydrophila. Based on morphological, biochemical and phylogenetic analysis, the isolate was identified as A. hydrophila. The antibiotic sensitivity test showed N201007 was hypersensitive to Doxycycline, Chloramphenicol, Enrofloxacin, Ciprofloxacin and Rocephin.