Abstract:

Phytohormones were a series of trace organic compounds synthesized in plants, which played an important role in regulation on plant growth and development, but occured in extremely low concentrations. The endogenous hormones determination technology was very important tool to study their regulatory activity in the plant life. There had been a great deal of discussion on the pretreatment and determination methods with regard to main plant hormones, including biological assay, physical and chemical assay and immunoassay in this paper, as well as the challenges and prospects in the determination methods of plant hormones had been discussed. Gas chromatography-mass spectroscopy and High Performance Liquid Chromatography detection methods were currently the mainstream methods of precise determination, but which needed higher pretreatment method. Liquid Chromatography-tandem mass spectometry had emerged as an effective method for hormones quantification because it combined efficient separation in Liquid Chromatography with selectivity and specificity in MS/MS.