Abstract:

Denaturing gradient gel electrophoresis (DGGE), which has advantages of high reliability, easy operation, and multiple samples to be processed simultaneously, has become a commonly used molecular technique for rapid fingerprint analysis in microbial community composition, diversity, population dynamics study and so on. Here, a background to the theory and progress of DGGE and its application in soil microbial ecological aspects were reviewed. Furthermore, the advantages and limitations of this technique in the study on microbial ecology were discussed and evaluated. Finally, its application potential was prospected.