Abstract:

To study the genetic transformation of Chinese cabbage-pe-tsai (Brassica compestris L. ssp. pekinensis Olsson ‘Jinyu60’), its tissue culture system was established. Cotyledons with petiole were used as explants, and the culture conditions were optimized by an orthogonal design. The results showed that, before and after genetic transformation, the optimum organization culture medium combination for ‘Jinyu60’ was: MS+3 mg/L 6-BA+0.3 mg/L NAA+4 mg/L AgNO3+2% sucrose+0.8% agar and MS+3 mg/L 6-BA+0.01 mg/L NAA+4 mg/L AgNO3+2% sucrose+0.8% agar, respectively. The concentration rate between 6-BA and NAA was 3:0.01.