Abstract:

The present study was carried out to evaluate possibility of application combination of Bacillus subtilus with intercropping fruit trees in pomegranate plantation to control pomegranate wilt pathogen Ceratocystis fimbriata. The role of strains of B. subtilis BS9601-Y1 and Y2 on the inhibition of C. fimbriata and leaves raw ethanol extracts of Eriobotrya japonica, Punica granatum, Zizyphus jujube, Eucalyptus spp were examined in laboratory condition. The results showed that the two strains of B. subtilis had strong inhibitory effect on C. fimbriata. The effect of Y2 was higher than Y1. The role of leaves of Z. jujube and E. japonica in inhibiting of C. fimbriata growth was stronger than other plants. The inhibition ratio of Z. jujube and E japonica were 29.1% and 44.6%, respectively. All leaves raw ethanol extracts showed strong inhibitory effect on B. subtilis. 0.5 mg/mL leaves extracts could inhibit 98% B. subtilis growth. In a word, the leaves extracts of trees planted in pomegranate yard influenced colonization and multiplication of B. subtilis. Therefore, intercropping fruit trees in pomegranate yard prevented using B. subtilis to control pomegranate wilt.