Abstract:

The enzyme activities of the rapeseed cytoplasmic male sterile lines of 09A and fertile lines of 09B , including peroxidase(POD), catalase(CAT), superoxide dismutase(SOD) and ascorbic acid peroxidase(APX) of different development stages of bud, were assayed by using spectrophotometry, the relationship between four kinds of protective enzyme activity and cytoplasmic infertility were investigated in this paper. At the same time, the content of malondialdehyde (MDA) and free proline were also assayed by spectrophotometry. The results indicated that along with the development of the bud, the enzyme activities of CAT, SOD, and APX were lower than fertile lines. POD activity was lower than fertile lines in the early development of the bud, but with the development of the bud growth to later period, POD activity of fertile lines of 09B gradually declined, and the POD activity of cytoplasmic male sterile was increasing gradually, and higher than 09B at last. In the cytoplasmic male sterile lines 09A, during the development of the bud, the content of the MDA were always higher than 09B, but the content of proline were lower than the fertile lines 09B.