Abstract:

It was important to detect transgene integration site after establishment of transgenic animal line, and it was necessary to determine transgene expression and function. The integration site was successfully amplified using Thermal Asymmetric Interlaced PCR (TAIL-PCR). The results showed that the target sequence was integrated into chromosome 12 (NW_003104315.1). The homozygosis analysis of integration site showed the transgenic bovine was heterozygosity. The transplanted gene could be easily and quickly located in the genomic sequence and TAIL-PCR has advanced future application.