Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (17): 87-91.doi: 10.11924/j.issn.1000-6850.2011-3756
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Abstract:
To establish a simple RP-HPLC method for determination the content of terberine hydrochloride in Modified Pulsatilla granule. HPLC method was used. The HPLC system consisted of Ture Kromasil C18 column (4.6 mm×250 mm, 5 μm) and acetonitrile-0.03 mol/L phosphoric acid (30:70, v:v) mixture as the mobile phase,with a flow rate of 1.0 mL/min. The detection wavelength was 265 nm. The column temperature was maintained at 30℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 93.00-186.00 μg/mL (r=0.9997, n=6). The average recovery was 99.49%, and RSD was 0.76% (n=6). The established approach was specific, accurate, reliable, prompt, sensitive and applicable. It can be used to control the quality of Modified Pulsatilla granule.
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中图分类号:R917
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2011-3756
https://www.casb.org.cn/EN/Y2012/V28/I17/87