Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (23): 85-89.doi: 10.11924/j.issn.1000-6850.2012-1193
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Abstract:
To develop a rapid and accurate real-time PCR method for detection of Listeria monocytogenes，hlyA gene of Listeria monocytogenes was used as target sequence to design primers.A new real-time PCR assay was developed. The standard curve was Y = -3．311X + 38.162，R2 =0.991． A real-time PCR assay for the detection of Listeria monocytogenes was developed，which supplied a technology platform for the rapid detection of Listeria monocytogenes．
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.2012-1193
https://www.casb.org.cn/EN/Y2012/V28/I23/85
