Abstract:

In order to identify Oryza Species at the molecular level，a total nine species of thirty-two plant materials were amplified the barcode gene atpF-atpH and sequenced the PCR products. Comparative sequencing of the atpF-atpH gene was carried out in order to design a specific primers for Oryza species .A phylogenetic tree which related species was outgroup was created. The results were as follows: The 113bp target fragment was amplified by the PCR from the Oryza Species materials,but other plant materials not Oryza Species were not amplified.According to phylogenetic analyses,we found the work flow could distinguish Oryza species from tested materials. It shows that based on the analysis of sequence difference of barcode genes，specific molecular markers can be used for the identification of Oryza Species.It probides detection methods for precious species identification at port.