Abstract:

The extraction of L-canavanine and the best reaction conditions of L-canavanine and 2,4-dinitrofluorobenzene in pH 9.5 borax buffer were studied in this paper, which can optimization the method of determination. The OD value of L-canavanine and 2,4-dinitrofluorobenzene’s complex in 571 nm was determined by spectrophotometry. The results showed that the best extraction method of L-canavanine was water extraction. When the volume of DNFB-acetonitrile was 30 μL, the complex reaction absorption value of L-canavanine and DNFB in 571 nm was maximum. The OD value of the complex was increasing with the extension of time after stability within 30 minutes. Corresponding linear regression equation was y=0.0083x-0.052; Correlation coefficient was R2=0.9986.