Abstract:

To study reasons for difference in content of the total flavonoid in different areas,and to reveal the reason from enzymes about difference of active ingredients of medicinal plants. Pick the wild Erigeron breviscapus from different areas in Yunnan Province, and put them back to laboratory alive and store in 4℃. Set out the activities of enzymes from fresh leaves,and triturate the remainder use to analyse the total flavonoid content. Based on the comparison between the total flavonoid content and the activities of enzymes of plenylalanine ammonia lyase (PAL) and 4-coumarate coenzyme A ligase(4CL) in Erigeron breviscapus from different areas. There were variations in total flavonoid content and the activities of enzymes of E. breviscapus growing in different producing areas, and total flavonoid content in the lowest content producing areas were 20.47%, the lowest content producing areas of the total flavonoid content and the activities of enzymes of PAL and 4CL are in Kunming, and positively correlation among them. The content of flavonoids in plants growing in different areas varied with differences in their synthesis, but there are other reasons affect the content of flavonoids in Erigeron breviscapus from different areas.