Abstract:

in this experiment, many new miRNAs in Echinococcus granulosus were discovered by high-throughout sequencing technology Solexa. To investigate the expression level of these miRNAs in different life stages of Echinococcus granulosus, Real-time PCR was used to relatively quantify the dominantly expressed miRNAs in protoscolex and germinal layer by combination of SYBR Green PCR and Race kit. It was found that mir-71, mir-7, mir-1and mir-19 were highly expressed at germinal layer other than protoscolex and let-7 was more aboudunt in protoscolex than germinal layer. The results suggested that miNRAs might play an important role in regulation of genes transcription in different developing stages of Echinococcus granulosus.