Abstract:

In order to establish specific and sensitive detection methods of porcine epidemic diarrhea virus (PEDV). A pair of primer was designed to amplify a product about 680 bp, according to the sequence of the M gene sequence of PEDV-CV777 strain of PEDV in Genbank. The amplified product was sequenced. Then the homology comparisons among the obtained sequence and the M Gene of the CV777 and SH5 stains were performed. The results showed that it shared 99.2% and 97.6% homology with M Gene of the CV777 and SH5, respectively. This method could be used to test PEDV, and the minimum detectable concentration of the PEDV RNA was 100 pg/μL. The method has been used to detect 58 portion specimens of diarrheal swine from three areas named A, B, and C in Fujian province, and the positive tested rate of PEDV was 87.9%.