Abstract:

The purpose of this study was to study the ovule-specific expression of the transgenic plants. Genomic DNA was extracted from Arabidopsis by using CTAB method, 35S::pCAMBIA1304-L117 gene plant expression vector was contructed, and then the genetic transformation of tomato was carried out. According to the reported INO promoter sequence, a pair of special primers was designed and synthesized. They were used to amplify by PCR, and this fragment was inserted into vector and sequenced. The results showed the cloned fragment had a homology of 100% with the INO promoter logged on GenBank. Furthermore the plant expression vector of pCAMBIA1304-L117 replacing the CAMV35S promoter with INO was constructed and transformed into agrobacterium EHA105 by the freezing method.The 35S::pCAMBIA1304-L117 gene plant expression vector was constructed,and it will lay a more foundation.