Abstract:

This paper was designed to discuss the mutation of single cells in potato by 60Co-γ irradiation and obtain potato mutants fast and efficient. The base of this experiment was used plantlet and tuber of purple potato variety ‘Heimeiren’ as material for callus induction and secondary culture to establish suspension culture line. Then single cells were selected as irradiation materials and 5 treatments with different radiation doses were designed to observe the regeneration rate of single cell in different radiation doses. The results indicated that, a certain amount of regenerated individual plantlets were produced with the radiation dose of 10 Gy while the regenerated individual plantlets were slower and lower with the radiation dose of 20 Gy. When the radiation dose was above 40 Gy there was no regenerated individual plantlets produced. The results of nucleus slices observation showed that, after the regeneration culture of 30 days, the change of nucleus shape was significantly and the effects of radiation dose of 10 Gy on nucleus were lower than that of 20 Gy. In conclusion, the mutation of single cells by 60Co-γ irradiation has certain stability and mutant materials have been expected to obtain.