Abstract:

This experiment was aimed to investigate the expression of Wnt-3a on the pre-implantation of both mouse parthenogenetic and normal embryos. In this study, Wnt-3a protein and mRNA be detected and analyzed by laser scanning confocal microscope and semi-quantitative RT-PCR. The results of laser scanning confocal microscope were shown that Wnt-3a protein was only detected on trophoblast cell of both normal and parthenogenetic embryos. The results of semi-quantitative RT-PCR were shown that the transcription level of Wnt-3a have no distinct difference between normal and parthenogenetic embryos. Collectively, it could be concluded that there was expression of Wnt-3a on both normal and parthenogenetic embryos, and there exists no distinct difference of transcription level between normal and parthenogenetic embryos.