Abstract:

To prepare Monoclonal Antibodies against Feline Calicivirus and identify their basic biological characteristics. By saturated ammonium sulfate method, differential centrifugation and chlorinated cesium density gradient centrifugation to Purify Feline Calicivirus (FCV), BALB/c mice were immunized by the purified Feline Calicivirus, the hybridoma cells secreting anti-feline Calicivirus monoclonal antibody were established by cell fusion and hybridoma screening technique. The results showed that: two Hybridoma Cells which could stably secret Monoclonal Antibody against FCV were obtained and named D8 and E5, the isotype of both two monoclonal antibodies was IgM, the two monoclonal antibodies had no cross-reaction with canine parvovirus (CPV), Feline Panleukopenia Virus (FPV) and Canine Distemper Virus (CDV). Successfully prepared the Monoclonal Antibodies against Feline Calicivirus, which laid the foundation for the establishment of relevant diagnostic method.