Abstract:

High-throughput sequencing is a major breakthrough in DNA sequencing technology development, the emergence of high-throughput sequencing technology provided an unprecedented opportunity to modern agricultural scientific research. This paper summarized the principle of the 454, Solexa and SOLiD second-generation high-throughput sequencing technology and the latest progress of the HeliScope and Pacific Biosciences SMRT, as represented by the single-molecule sequencing technology. On this basis, the high-throughput sequencing technology was summed up in genome sequencing, transcriptome sequencing, small RNA molecules and digital gene expression profiles and other research areas in agricultural research. Finally, the characteristics and development prospect of high-throughput sequencing technology was noticed.