Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2012, Vol. 28 ›› Issue (30): 254-258.doi: 10.11924/j.issn.1000-6850.2012-0679

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Composite mutation breeding of antagonistic bacteria GF-312

  

  • Received:2012-03-02 Revised:2012-04-23 Online:2012-10-25 Published:2012-10-25

Abstract:

In order to improve the antibacterial activity of antagonistic bacteria has been screened, as a initial strain, Pseadomonas fluorescens GF-312 was mutated by UV and MW. Based on the lethal rate, the positive mutation rate and the antagonistic activity, the best optional time of UV and microwave irradiation were determined. Combining with the genetic stability of strains selected, the high efficient strain named GF-16 was obtained. The result of antifungal activity showed that the diameter of inhibition reached up to 30.4 mm, and the activity increased by 18.8%.