Abstract:

In order to establish a method SDS-PAGE and Western-blot suitable for heat shock protein study of Lactuca sative L., with leaves of Lactuca sative L. (W7) as material, we used improved TCA/Acetone method extracted leaf protein, compared protein separating effect in SDS gel in two different volt, obtained clear bands of HSP70 and rubisco using anti-body diluent stored 2 weeks at 4℃, optimized and preliminary established Western-blot experiment condition of heat shock protein of Lactuca sative L. young leaves.