Abstract:

The aim of this study are to improve the utilization rate of resources in cheap tea, and to solve the prolem of using chloroform, ethyl acetate and other toxic organic solvent in tea polyphenols production process, which is easy to cause environmental pollution. With cheap tea as the raw material, adopting the safe edible ethanol and water solvent, the effect of static adsorption and de-sorption on tea polyphenols, catechins, and caffeine by BYX, HPD200A, HZ103, HZ841, NKA-9, PA, ADS17, XDA5, XDA7, NPS1, NPS2, HP20 resin. The BYX macro porous resin was screened from the twelve kinds of resins. The BYX macro porous resin was then applied for dynamic elution of tea polyphenols. The result showed that in the condition of 1400 mL BYX resin column, 37.5 g samples, 2 BV/h flowing velocity, 80% ethanol, 3 BV elution volume, the purity and yield of tea polyphenols could respectively reach to 98.36% and 19.56%, and the content of catechins and EGCG could respectively reach to 80.82%and 46.52%, however the content of caffeine was only 0.96%. The green preparation method of tea polyphenols was wieldy, safety and health. Any toxic organic solvents and harm pollutants were not used in all process, such as ethyl acetate, chloroform. It may provide one new way to green manufacturing producing high quality tea polyphenols.