Abstract:

For the study were provided the basis the genetic diversity, by comparing different enzyme cut method, set up and optimize the balsam pear AFLP reaction system, two kinds of AFLP reaction system with the different restriction enzyme combinations (TaqI/VspI and EcorI/MseI) were compared in Momordica charantia L.. The results show that the effect of EcorI/MseI enzymes is best one, polymorphic bands are clear and the augmentation signal strength are basically the same, 32 primers were screened preliminary with stable amplification and rich polymorphism. The most appropriate enzymes that cut method is the use of EcorI/MseI combination for double enzyme cut, through the repeat tests show that this system is stable and reliable, and can be used in the genetic diversity of Momordica charantia L..