Abstract:

The aim of this study was to evaluate the effects of strengths of MS medium, different culture temperatures and time on prevent explant browning in plants of Camellia sinensis cv Fuzao 2#. Axillary buds reserved a part of stalk excised from juvenile twigs origin of current year growth tea plants is explant. Explants cultured on basal medium supplemented with BA 2.0 mg?L-1 +IBA 0.1mg?L-1+sucrose 20g?L-1+agar 7.5g?L-1 after adsorbing phenolics used 2.0g?L-1 PVP solution for 1h. Minimum rate of explant browning (5.6%) were developed on 1/4MS at 10℃ under 48h dark conditions. The results showed no differences between strengths of MS medium and culture time in the percentage of explant browning, however, culture temperature have significant effect on explant browning. the effect of the factors on explant browning was in sequence of culture temperature>strengths of MS medium>culture time, low culture temperature is an essential factor in prevent explant browning. Successful reduces axillary buds browning in vitro culture by low temperature suggested that this strategy was viable for preventing explant browning.