Abstract: The characteristic of VvWRKY71 transcription factor in grape was analyzed and predicted by bioinformatic methods in the following aspects: physical and chemical characters, secondary and tertiary structure of protein, conserved domain, subcellular localization, function category and molecular phylogenetic evolution. The results showed that VvWRKY71 was an unstable hydrophilic proteins which made of 311 amino acid with molecular weight 34.8KDa; Random coil and α-helix were the major structural element of polypeptide chain; And it possessed one conserved WRKY domain, so it belonged to WRKY transcription factor; Subcellular localization result indicated that VvWRKY71 was located in nucleus very likely. Function analysis indicated that it was involved in transcription and transcription regulation. This research showed VvWRKY71 would play important function as a transcription factor.