Abstract: Field experiments were carried out to study effect of different fertilizer rate of soybean meal and nitrogen on greenhouse cucumber. Which included single application of soybean meal, chemical fertilizer and soybean meal application combine with organic fertilizer on cucumber yield, quality and the utilization ratio of the fertilizer and the soil fertility. The results showed that, single application of soybean meal or chemical fertilizer and combine application of soybean meal and chemical fertilizer could significantly increased the total yield of cucumber with the same nitrogen, phosphorus, potassium fertilizer application amount. Compared with treatment CK, the yield of M2N1, M1N1 and M1N2 treatments were increased by 47.1%, 68.6% and 30.5%, respectively. There was no significant difference between the different treatments in cucumber fruit soluble sugar, protein and nitrate, nitrite contents, but Vc content in M2N1 treatment was the highest. Different fertilization treatment could significantly improve cucumber absorption of nitrogen and potassium. But there was no significant difference in the nitrogen, phosphorus, potassium fertilizer utilization rate between all treatments. Soil inorganic nitrogen, nitrogen, phosphorus, potassium and pH value of soil fertility properties changed obviously after cucumber harvest. Soil pH and soil nutrient changes showed a negative correlation, the soil pH value and soil available phosphorus, rapidly available potassium content was significant negative correlation with soil, ammonium nitrogen, nitrate nitrogen and inorganic nitrogen content was negatively related to. Nitrogen, phosphorus, and potassium of soybean meal release rate was higher, could completely replace the common organic manure combined with chemical fertilizer for cucumber production, the preferred proportion of soybean meal nitrogen and chemical nitrogen fertilizer was 1:1 or 2:1.