Abstract: In this experiment, the effect of cytokinin on maize yield characteristics by spraying of exogenous different concentrations of cytokinin at heading was studied. The results indicated that, fresh weight of single ear maize showed stable after the first rise, compared with the control, which was significantly better; maize plants of cytokinin treatment were relatively taller, and had thicker stems, while the areas of ear leaves were larger, and chlorophyll and soluble protein content were also significantly increased, and productions too. And the best treatment was 8 mg/L cytokinin, compared with control, plant height, the ear nodal width increased by 10.3%, 14.5%, respectively. In addition, chlorophyll and soluble protein content improved by 11.9%, 49.9%. Therefore, kernle number per plant and 1000-kernel weight increased by 32.9% and 23.8%. These suggested that cytokinin could delay leaf senescence of maize and enhance lodging resistance and boost the field.