Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (4): 57-61.doi: 10.11924/j.issn.1000-6850.2012-2985

Special Issue: 园艺

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Isolation and Identification of Antagonistic Ginger Endophytic Bacteria for Aspergillus niger

  

  • Received:2012-09-03 Revised:2012-10-18 Online:2013-02-05 Published:2013-02-05

Abstract: In order to find a safe, mildew resistance endophytes to solve the problems of safe and cost of the use of chemical fungicides. Ginger was surface sterilized by using 70% ethanol, 10% sodium hypochlorite and 5% phenol. 13 endophytes were isolated from ginger by using gradient dilution and streak plate method. After repetitive co-culture on PDA plates, results showed that the growth of Aspergillus niger could be strongly inhibited by JT716. According to the morphology characteristics, physiology and biochemistry tests, the comparison of 16S rDNA sequence, JT716 was similar to Pseudomonas fluorescens.