Abstract: In order to more clear understanding of the Lanzhou city agricultural science and technology innovation ability and level, for Lanzhou city agricultural science and technology innovation ability construction to provide the corresponding analysis perspective and basis, based on the comprehensive evaluation index method and analytic hierarchy process, agricultural science and technology innovation ability of Lanzhou from 2007 to 2011 is used to evaluate by using the statistics of 2007-2011. The results showed that, the period of Lanzhou City agricultural science and technology innovation ability has been increased, at the end of 2011 agricultural science and technology innovation ability evaluation index was 0.622, and a trend of rising. And then were compared with those of the western region, found that agricultural science and technology innovation ability of Lanzhou city was weak in the west. Finally proposed Lanzhou should use its own advantages, pay attention to cultivating innovative talents, setting up enterprise independent innovation, attracting foreign investment, perfecting the technology market and agricultural science and technology innovation system and so on, so as to enhance the level of agricultural science and technology innovation ability.