Abstract: To further analyze the high- yielding qualities of hybrid rape under different fertilizer application levels, the experiment was conducted to screen an optimal basic number of fertilization in order to create favorable conditions for high-yielding and provide theoretical basis for large areas of rapeseed production to obtain a high yield.‘Yunyouza 10’, a new hybrid rape cultivar, was selected as materials to study effect of 5 different fertilizer application levels on the yield of hybrid rape under the same management condition from 2011 to 2012 Jinji Town of Longyang District in Baoshan, Yunnan Province. The results showed that plot yields were between 606.66 kg/hm² and 3222.5 kg/hm², the output was the highest when using 675 kg/hm² carbamide, 750 kg/hm² calcium superphosphate, 300 kg/hm² potassium sulphate and 22.5 kg/hm² boron fertilizer in the whole growing period, or 18.5% base fertilizer, 30% seedlings growth fertilizer, 34.3% strong seedling fertilizer and 17.2% peduncle growth fertilizer. In Longyang District and the climate similar hybrid rape high-yielding areas in Yunnan Province, the results might be useful for application in large area. If the controlled-release BB fertilizer nutrition was given out 20 days to 25 days ahead of time and increasing 150 kg/hm² under 600 kg/hm² the controlled-release BB fertilizer conditions, the anticipated high efficient results could be attained.